Title :
GP73 N-glycosylation at
Asn144 reduces hepatocellular carcinoma cell motility and invasiveness
Abstract :
- Golgi Protein 73 ( GP73 ) is a potential liver disease glycobiomarker warranting comprehensive analyses of its glycan structure and glycosylation function
- In this study, we used mass spectrometry to identify glycosylation sites and the glycan structure, high-throughput lectin microarray to provide rapid and sensitive profiling of glycoconjugates, and site-directed mutagenesis to clarify the impact of glycans on target glycoproteins in vivo
- We identified three GP73 N-glycosylation sites : Asn109, Asn144 and Asn398
- We found five glycoforms on Asn144 , including biantennary, triantennary and fucosylated glycans
- Removal of N-glycans at Asn144 enhanced the motility and invasiveness of hepatocellular carcinoma cells, possibly due to inhibition of cell adhesion related to the changes of cell membrane glycosylation
- This study increases our understanding of the functional relevance of GP73 glycosylation and suggests that Asn144-deleted GP73 can influence the progression and metastasis of hepatocellular carcinoma
Output (sent_index, trigger,
protein,
sugar,
site):
- 0. N-glycosylation, , -, -, Asn144
- 2. glycoproteins, , glycoproteins, -, -
- 2. glycosylation, , -, -, sites
- 3. N-glycosylation, , -, -, Asn109, Asn144 and Asn398
- 3. N-glycosylation, , -, -, sites
- 3. sites, , -, -, Asn109, Asn144 and Asn398
- 3. sites, , -, -, sites
- 4. fucosylated, , -, fucosylated glycans, -
- 4. glycoforms, , -, -, Asn144
- 5. Removal, , -, N-glycans, Asn144
Output(Part-Of) (sent_index,
protein,
site):
- 0. GP73 N-glycosylation, Asn144
- 3. GP73, Asn109, Asn144 and Asn398
- 3. GP73, sites
*Output_Site_Fusion* (sent_index,
protein,
sugar,
site):
- 0. GP73 N-glycosylation, -, Asn144
- 3. GP73, -, Asn109, Asn144 and Asn398
- 4. GP73 N-glycosylation, -, Asn144
- 5. GP73 N-glycosylation, N-glycans, Asn144