Title :
Ribosomal protein S3 (
rpS3 ) secreted from various cancer cells is N-linked glycosylated
Abstract :
- Ribosomal protein S3 ( rpS3 ) is a 243 amino acid component of the 40S ribosomal small subunit
- It has multiple roles in translation and extra-ribosomal functions like apoptosis and DNA repair
- RpS3 is secreted only in cancer cell lines
- Presently, mass spectrometry analysis revealed rpS3 to be glycosylated at the Asn165 residue
- A point mutation at this residue decreased secretion of rpS3 in cancer cell lines
- Secretion was also inhibited by the endoplasmic reticulum (ER)-Golgi transport inhibitor Brefeldin A and by Tunicamycin , an inhibitor of N-linked glycosylation
- N-linked glycosylation of rpS3 was confirmed as necessary for rpS3 secretion into culture media via the ER-Golgi dependent pathway
- RpS3 bound to Concanavalin A , a carbohydrate binding lectin protein, while treatment with peptide-N-glycosidase F shifted the secreted rpS3 to a lower molecular weight band
- In addition, the N165G mutant of rpS3 displayed reduced secretion compared to the wild-type
- An in vitro binding assay detected rpS3 homodimer formation via the N-terminal region ( rpS3 :1-85) and a middle region ( rpS3 :95-158)
- The results indicate that the Asn 165 residue of rpS3 is a critical site for N-linked glycosylation and passage through the ER-Golgi secretion pathway
Output (sent_index, trigger,
protein,
sugar,
site):
- 0. glycosylated, , Ribosomal protein S3, -, -
- 0. glycosylated, , rpS3, -, -
- 4. glycosylated, , -, -, Asn165 residue
- 7. glycosylation, , rpS3, -, -
- 8. Concanavalin, , Concanavalin A, a carbohydrate binding lectin protein, -
Output(Part-Of) (sent_index,
protein,
site):
- 11. rpS3, Asn 165 residue
- 11. rpS3, site
*Output_Site_Fusion* (sent_index,
protein,
sugar,
site):
- 4. Ribosomal protein S3, -, Asn165 residue