PMID: 2775174

 

    Legend: Gene, Sites

Title : Purification and characterization of rat liver glycosylasparaginase

Abstract :
  1. 1
  2. Rat liver glycosylasparaginase [ N4-(beta-N-acetylglucosaminyl)-L-asparaginase , EC 3.5.1.26] was purified to homogeneity by using salt fractionation, CM-cellulose and DEAE-cellulose chromatography, gel filtration on Ultrogel AcA-54, concanavalin A-Sepharose affinity chromatography, heat treatment at 70 degrees C and preparative SDS /polyacrylamide-gel electrophoresis
  3. The purified enzyme had a specific activity of 3.8 mumol of N-acetylglucosamine/min per mg with N4-(beta-N-acetylglucosaminyl)-L- asparagine as substrate
  4. 2
  5. The native enzyme had a molecular mass of 49 kDa and was composed of two non-identical subunits joined by strong non-covalent forces and having molecular masses of 24 and 20 kDa as determined by SDS /polyacrylamide-gel electrophoresis
  6. 3
  7. The 20 kDa subunit contained one high-mannose-type oligosaccharide chain, and the 24 kDa subunit had one high-mannose-type and one complex-type oligosaccharide chain
  8. 4
  9. N-Terminal sequence analysis of each subunit revealed a frayed N-terminus of the 24 kDa subunit and an apparent N-glycosylation of Asn-15 in the same subunit
  10. 5
  11. The enzyme exhibited a broad pH maximum above 7
  12. Two major isoelectric forms were found at pH 6.4 and 6.6
  13. 6
  14. Glycosylasparaginase was stable at 75 degrees C and in 5% (w/v) SDS at pH 7.0
Output (sent_index, trigger, protein, sugar, site):
  • 7. contained, , subunit, one high-mannose-type oligosaccharide chain, -
  • 7. had, , subunit, complex-type, -
  • 7. had, , subunit, high-mannose-type, -
  • 9. N-glycosylation, , subunit, -, -
  • 9. N-glycosylation, , subunit, -, Asn-15
Output(Part-Of) (sent_index, protein, site):
*Output_Site_Fusion* (sent_index, protein, sugar, site):
  • 9. subunit, -, Asn-15

 

 

Protein NCBI ID SENTENCE INDEX