PMID: 28514451

 

    Legend: Gene, Sites

Title : Structure of the full-length glucagon class B G-protein-coupled receptor

Abstract :

1. The human glucagon receptor , GCGR , belongs to the class B G-protein-coupled receptor r family and plays a key role in glucose homeostasis and the pathophysiology of type 2 diabetes
2. Here we report the 3.0 Å crystal structure of full-length GCGR containing both the extracellula r domain and transmembrane domain in an inactive conformation
3. The two domains are connected by a 12-residue segment termed the stalk, which adopts a β-strand conformation, instead of forming an α-helix as observed in the previously solved structure of the GCGR transmembrane domain
4. The first extracellula r loop exhibits a β-hairpin conformation and interacts with the stalk to form a compact β-sheet structure
5. Hydrogen-deuterium exchange, disulfide crosslinking and molecula r dynamics studies suggest that the stalk and the first extracellula r loop have critical roles in modulating peptide ligand binding and receptor activation
6. These insights into the full-length GCGR structure deepen ou r understanding of the signalling mechanisms of class B G-protein-coupled receptors

Output (sent_index, trigger, protein, sugar, site):

Output(Part-Of) (sent_index, protein, site):

• 2. GCGR, domain
• 2. r, domain
• 3. structure of the GCGR, domain

*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

Protein	NCBI ID	SENTENCE INDEX
glucagon	2641	0
structure of the GCGR	2642	3
r	10663	1
GCGR	2642	1,2,6
glucagon receptor	2642	1
G-protein-coupled receptor	10663	1