PMID: 3200844

 

    Legend: Gene, Sites

Title : Partial cDNA sequence encoding a nuclear pore protein modified by O-linked N-acetylglucosamine

Abstract :
  1. The nuclear pore complex contains a family of proteins ranging in molecular mass from 35 to 220 kDa that are glycosylated with O-linked N-acetylglucosamine (GlcNAc) residues
  2. We sought to determine the primary sequence of a nuclear pore protein modified by O-linked GlcNAc
  3. The major (62 kDa) nuclear pore glycoprotein ( np62 ) was purified from rat liver nuclear envelopes by immunoaffinity chromatography and preparative gel electrophoresis
  4. After CNBr fragmentation , a glycopeptide was isolated and microsequenced
  5. An oligonucleotide probe based on this sequence information was used to screen a lambda gt11 cDNA library constructed from poly(A) mRNA of the rat thyroid cell line FRTL-5
  6. A clone (B5) was isolated and shown to hybridize to a single 2.5-kilobase species in poly(A) mRNA from rat liver and FRTL-5
  7. This insert was sequenced and found to contain a 691-base-pair cDNA encoding a 155-amino acid open reading frame
  8. This open reading frame contained a CNBr fragment identical to the original glycopeptide sequence and a second CNBr fragment corresponding to a nonglycosylated peptide that was also isolated from the purified pore glycoprotein
  9. The B5 cDNA produced a beta-galactosidase fusion protein of the size predicted by the open reading frame
  10. Analysis of the residues making up a presumptive glycosylation site suggests that the sequence is unlike any known sites for enzymatic N- or O-linked glycosylation
  11. The partial sequence of the 62-kDa nuclear pore glycoprotein shows little similarity to other characterized proteins and elucidates structural features of a member of the family of nuclear pore glycoproteins
Output (sent_index, trigger, protein, sugar, site):
  • 10. glycosylation, , -, -, site
  • 11. glycoprotein, , glycoprotein, -, -
  • 11. glycoproteins, , glycoproteins, -, -
  • 3. glycoprotein, , glycoprotein, -, -
  • 3. glycoprotein, , np62, -, -
  • 4. glycopeptide, , -, -, glycopeptide
  • 8. glycopeptide, , -, -, glycopeptide sequence
  • 8. glycoprotein, , glycoprotein, -, -
  • 8. nonglycosylated, , -, -, peptide
Output(Part-Of) (sent_index, protein, site):
  • 11. glycoprotein, sequence
  • 2. protein, sequence
  • 8. CNBr, fragment
*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

 

Protein NCBI ID SENTENCE INDEX
np62 65274 3