Title : Site specific glycosylation patterns of
H-2K : effects of allelic polymorphism and mitogenic stimulation
Abstract :
- The site-specific glycosylation patterns of two H-2K alleles, k and b, were determined on splenic T cells metabolically labeled with [3H]mannose
- Cells from B10, B10.A, (B10 X B10.A)F1, and C3H mice were examined, along with the effect of short- (8 hr) and long-term (36 hr) mitogenic stimulation
- For both glycosylation sites ( Asn86 and Asn176 ) of both antigens, 80% of the structures consisted of mono- and bisialylated biantennary N-linked complex oligosaccharides, with the remaining consisting of smaller (probably high mannose) structures
- Asn176 of both H-2Kk and H-2Kb contained the same ratio (2.8 to 1) of bi- to monosialylated chains
- However, Asn86 of H-2Kb contained a higher ratio (5 to 1), while Asn86 of H-2Kk a lower ratio (1.5 to 1)
- This difference was seen on antigens isolated from cells of the parental strains as well as from the F1 cross
- The glycosylation of H-2Kk did not vary between B10.A and C3H mice
- Mitogenic stimulation increased markedly both total [3H]mannose incorporation and the spectrum of N-linked oligosaccharides labeled
- For H-2Kk , it had no effect on sialylation, but resulted in a slight under galactosylation of the monosialylated structures at both sites
- A comparison of the patterns seen here, determined on nontransformed T cells, with those previously determined on H-2Kk from a B lymphoma line, revealed marked differences in sialylation and branching patterns at both sites
- These data indicate that glycosylation differences may be found between highly homologous (91%) alleles of an H-2 gene, even when co-dominantly expressed by F1 cells; however, the patterns do change with mitogenic stimulation, and between normal and transformed cells
Output (sent_index, trigger,
protein,
sugar,
site):
- 0. glycosylation, , H-2K, -, -
- 3. bisialylated, , -, bisialylated biantennary N-linked complex oligosaccharides, -
- 3. glycosylation, , -, -, Asn86 and Asn176
- 3. glycosylation, , -, -, sites
- 3. sites, , -, -, Asn86 and Asn176
- 3. sites, , -, -, sites
- 4. monosialylated, , -, monosialylated chains, -
- 7. glycosylation, , H-2Kk, -, -
- 9. monosialylated, , -, the monosialylated structures, -
Output(Part-Of) (sent_index,
protein,
site):
- 4. H-2Kb, Asn176
- 4. H-2Kk, Asn176
- 5. H-2Kb, Asn86
- 5. H-2Kk a, Asn86
*Output_Site_Fusion* (sent_index,
protein,
sugar,
site):
- 3. H-2K, -, Asn86 and Asn176