PMID: 3555489

 

    Legend: Gene, Sites

Title : Molecular cloning and amino acid sequence of rat enkephalinase

Abstract :

1. cDNA clones encoding rat enkephalinase ( neutral endopeptidase , EC 3.4.24.11) have been isolated in lambda gt10 libraries from both brain and kidney mRNAs and the complete 742 amino acid sequence of rat enkephalinase is presented
2. The enzyme possesses a single transmembrane spanning domain near the N-terminal of the molecule but lacks a signal sequence
3. Because enkephalinase has it active site located extracellularly and is thus an ectopeptidase, we suggest that the N-terminal transmembrane region of the enzyme anchors the protein in membranes and that the majority of the protein, including the carboxy terminus , is extracellular
4. Enkephalinase , a zinc-containing metallo enzyme , displays homology with other zinc metallo enzymes such as carboxypeptidase A, B and E , suggesting enzymatic similarities in these enzymes

Output (sent_index, trigger, protein, sugar, site):

Output(Part-Of) (sent_index, protein, site):

• 0. enkephalinase, sequence
• 1. enkephalinase, sequence
• 2. enzyme, domain
• 3. Because enkephalinase, site
• 3. enzyme, region

*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

Protein	NCBI ID	SENTENCE INDEX
Because enkephalinase	24590	3
carboxypeptidase A, B and E	24271	4
enkephalinase	24590	0,1
Enkephalinase	24590	4