Title : The carbohydrate moiety of human platelet
glycocalicin : the structures of the major
Asn-linked sugar chains
Abstract :
- Glycocalicin , a predominant glycoprotein on the human platelet surface, has been purified from a platelet suspension by means of sonication, ammonium sulfate precipitation and acid treatment followed by chromatography on columns of wheat germ agglutinin-Sepharose and Mono Q. Asparagine-linked (N-linked) oligosaccharides were released by hydrazinolysis, and then N-acetylated and reduced with NaBH4 or NaB3H4
- The released carbohydrate chains were found to be of the complex-type from their interaction with immobilized lectin columns
- The structures of the two major oligosaccharide-alditols separated by ion-exchange chromatography on a Mono Q column were investigated by means of methylation analysis, glycosidase digestion, and Smith periodate degradation, and they were assigned as typical di- and trisialylated complex-type oligosaccharide-alditols with two and three peripheral chains consisting of Gal-GlcNAc sequences, respectively
Output (sent_index, trigger,
protein,
sugar,
site):
- 0. Asn-linked, , -, the major Asn-linked sugar chains, Asn
- 0. glycocalicin, , glycocalicin, The carbohydrate moiety, -
- 1. Asparagine-linked, , -, wheat germ agglutinin-Sepharose and Mono Q. Asparagine-linked (N-linked) oligosaccharides, Asparagine
- 1. glycoprotein, , Glycocalicin, -, -
- 1. glycoprotein, , glycoprotein, -, -
- 3. trisialylated, , -, trisialylated complex-type oligosaccharide-alditols, -
Output(Part-Of) (sent_index,
protein,
site):
*Output_Site_Fusion* (sent_index,
protein,
sugar,
site):