PMID: 3651384

 

    Legend: Gene, Sites

Title : Biosynthesis and molecular cloning of sulfated glycoprotein 2 secreted by rat Sertoli cells

Abstract :
  1. Sulfated glycoprotein 2 ( SGP-2 ) is the major protein secreted by rat Sertoli cells
  2. Pulse-chase labeling shows that SGP-2 is synthesized as a cotranslationally glycosylated 64-kDa precursor that is modified to a negatively charged 73-kDa form before intracellular cleavage to the mature 47- and 34-kDa subunits
  3. A plasmid cDNA library was constructed from immunopurified mRNA, and a recombinant clone containing the entire protein coding sequence of SGP-2 was isolated
  4. The 1857-nucleotide cDNA consists of a 297-nucleotide 5' noncoding segment, a 1341-nucleotide coding segment, and a 219-nucleotide 3' noncoding sequence
  5. The 5' noncoding region contains five ATG codons followed by four short open reading frames
  6. The derived SGP-2 sequence has a molecular weight of 51,379 and contains six potential N-glycosylation sites
  7. Proteolytic processing sites for the prepro protein were determined by amino-terminal sequencing of the isolated SGP-2 subunits
  8. Northern blots show a wide tissue distribution for the 2.0-kb SGP-2 message, and computer sequence analysis indicates a significant relationship between SGP-2 and human apolipoprotein A-I
Output (sent_index, trigger, protein, sugar, site):
  • 0. glycoprotein, , sulfated glycoprotein 2, -, -
  • 1. glycoprotein, , SGP-2, -, -
  • 1. glycoprotein, , Sulfated glycoprotein 2, -, -
  • 1. glycoprotein, , protein, -, -
  • 2. glycosylated, , SGP-2, -, -
  • 2. glycosylated, , precursor, -, -
  • 6. N-glycosylation, , -, -, sites
Output(Part-Of) (sent_index, protein, site):
  • 3. SGP-2, sequence
  • 3. protein, sequence
  • 6. -, sites
  • 6. SGP-2, sequence
*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

 

Protein NCBI ID SENTENCE INDEX
apolipoprotein A-I 335 8