PMID: 3934016

 

    Legend: Gene, Sites

Title : Processing of rat and human angiotensinogen precursors by microsomal membranes

Abstract :

1. We have studied the processing of rat and human angiotensinogen precursors by microsomal membranes as a means of determining the number of asparagine-linked oligosaccharide units per angiotensinogen molecule, and thus the utilization of potential sites of N-glycosylation
2. Glycosylated, processed forms of angiotensinogen were isolated by chromatography on lentil lectin-Sepharose 4B
3. 35S- Methionine-labeled precursor and processed forms of angiotensinogen were compared with glycosylated and nonglycosylated 35S- methionine-labeled mature forms of angiotensinogen secreted by hepatoma cells, using immunoprecipitation, sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography
4. N-Glycosylation of secreted angiotensinogen was inhibited using tunicamycin
5. For rat angiotensinogen , only 2 of 3 potential sites of N-glycosylation were utilized; in contrast, all 4 potential sites of N-glycosylation of human angiotensinogen were utilized
6. For neither rat or human angiotensinogen precursor was there any evidence for a prosequence

Output (sent_index, trigger, protein, sugar, site):

• 1. N-glycosylation, , -, -, sites
• 1. asparagine-linked, , -, asparagine-linked oligosaccharide units, asparagine
• 3. glycosylated, , angiotensinogen, -, -
• 3. nonglycosylated, , angiotensinogen, -, -
• 4. N-Glycosylation, , angiotensinogen, -, -
• 5. N-glycosylation, , -, -, sites
• 5. N-glycosylation, , angiotensinogen, -, -
• 5. utilized, , -, -, sites

Output(Part-Of) (sent_index, protein, site):

*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

Protein	NCBI ID	SENTENCE INDEX
angiotensinogen	183	1,2,3,4,5
angiotensinogen precursors	183	0,1
angiotensinogen precursor	183	6