PMID: 4077844

 

    Legend: Gene, Sites

Title : Structures of sugar chains of the third component of human complement

Abstract :

1. Human C3, the third component of human complement, contained mannose and N-acetylglucosamine as sugar components
2. The sugar chains were liberated from the polypeptide chains by hydrazinolysis, and the free amino groups were N-acetylated
3. The reducing end residues of the sugar chains thus obtained were tagged with 2-aminopyridine, and the pyridylamino (PA-) derivatives of sugar chains were separated by high-performance liquid chromatography
4. The structures of purified PA-sugar chains were analyzed by a combination of stepwise exoglycosidase digestions, size determination by paper electrophoresis, methylation analysis, Smith degradation, and partial acetolysis
5. These results showed that C3 contained two high-mannose type sugar chains ranging from Man5GlcNAc2 to Man9GlcNAc2
6. Analyses of the sugar chains of alpha- and beta-chains of C3 indicated that the alpha-chain contained mainly Man8GlcNAc2 and Man9GlcNAc2, while the beta-chain contained mainly Man5GlcNAc2 and Man6GlcNAc2

Output (sent_index, trigger, protein, sugar, site):

• 6. contained, , alpha-chain, Man8GlcNAc2, -
• 6. contained, , alpha-chain, Man9GlcNAc2, -
• 6. contained, , beta-chain, Man5GlcNAc2, -
• 6. contained, , beta-chain, Man6GlcNAc2, -

Output(Part-Of) (sent_index, protein, site):

*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

Protein	NCBI ID	SENTENCE INDEX