PMID: 479158

 

    Legend: Gene, Sites

Title : Carbohydrate of the human plasminogen variants

Abstract :
  1. III
  2. Structure of the O-glycosidically linked oligosaccharide unit
  3. The preceding two manuscripts in this issue (Hayes, M. L., and Castellino, F. J. (1979) J. Biol
  4. Chem
  5. 254, 8768-8771, 8772-8776) describe the isolation and characterization of glycopeptides from human plasminogen affinity chromatography variants 1 1 and 2
  6. Plasminogen variant 1 1 contains an asparagine288-based branched carbohydrate structure, which has been established in the immediately preceding manuscript
  7. This structure is absent in variant 2
  8. Plasminogen variants 1 1 and 2 contain a threonine-based glycoconjugate
  9. This latter structure has been established by combination of methylation data, glycosidase digestions, periodate oxidations, and Smith degradations of the beta-eliminated reduced oligosaccharides
  10. One glycopeptide unit, isolated from both plasminogen variants 1 1 (1D) and 2 (2D) possessed the following structure: Sia alpha 2 yields 3Gal beta 1 yields 3GalNAc- Thr
  11. The threonine was found to be residue 345 in the Glu- plasminogen sequence
  12. Another glycopeptide unit was also found to be present, in lower yields in both variants 1 1 (1E) and 2 (2E)
  13. The structure of this unit was: Sia alpha 2 yields 3Gal beta1 yields 3GalNAc- Thr
  14. : formula: (see text), alpha 2,6 Sia
  15. Again Thr 345 was the glycosylated amino acid
  16. The amino acid sequence around the glycosylated threonine was found to be NH2- Ala
  17. Pro.Thr(CHO)
  18. Ala.Pro.Pro.Glu
Output (sent_index, trigger, protein, sugar, site):
  • 0. variants, , plasminogen variants, Carbohydrate, -
  • 10. glycopeptide, , -, -, glycopeptide
  • 12. glycopeptide, , -, -, glycopeptide
  • 15. glycosylated, , -, -, Thr 345
  • 16. glycosylated, , -, -, threonine
  • 5. glycopeptides, , -, -, glycopeptides
  • 6. asparagine288-based, , -, an asparagine288-based branched carbohydrate structure, asparagine288
  • 6. contains, , Plasminogen variant 1, an asparagine288-based branched carbohydrate structure, -
Output(Part-Of) (sent_index, protein, site):
  • 11. plasminogen, sequence
*Output_Site_Fusion* (sent_index, protein, sugar, site):
  • 15. plasminogen variants, -, Thr 345
  • 6. Plasminogen variant 1, an asparagine288-based branched carbohydrate structure, asparagine288

 

 

Protein NCBI ID SENTENCE INDEX