PMID: 6327299

 

    Legend: Gene, Sites

Title : Structural studies on the O-linked carbohydrate chains of human platelet glycocalicin

Abstract :

1. Glycocalicin (140 kDa), constituting the main part of glycoprotein Ib (160 kDa), was released from the human platelet membrane by the action of a Ca2 +-dependent protease , present in the platelet cytoplasm and liberated during sonication of the platelet suspension
2. After activation of the protease by Ca2 +, the sonicated platelet suspension was subjected to differential centrifugation
3. The supernatant was applied to a column of wheat germ agglutinin linked to Sepharose 4B; glycocalicin was eluted from the column with 2.5% (w/v) N-acetylglucosamine
4. Glycocalicin was found to contain 40% carbohydrate by weight, representing N- as well as O-glycosidically linked carbohydrate chains
5. The O-glycosidic chains were split off by alkaline cleavage in the presence of 3H-labelled NaBH4
6. The liberated 3H-labelled oligosaccharide-alditols were fractionated on a DEAE-Sephadex A-25 column
7. The structures of the oligosaccharide-alditols were investigated by 500-MHz 1H-NMR spectroscopy
8. The major compound was identified as NeuAc alpha(2----3)Ga1 beta(1----3)[NeuAc alpha(2----3)Ga1 beta(1----4)GlcNAc beta(1----6)]GalNAc-ol
9. Two minor compounds were found to be NeuAc alpha(2----3)Gal beta(1----3)[NeuAc alpha(2----6)]GalNAc-ol and NeuAc alpha(2----3)Gal beta(1----3)GalNAc-ol

Output (sent_index, trigger, protein, sugar, site):

• 0. glycocalicin, , glycocalicin, the O-linked carbohydrate chains, -
• 1. glycoprotein, , glycoprotein, -, -
• 4. contain, , Glycocalicin, 40% carbohydrate, -

Output(Part-Of) (sent_index, protein, site):

*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

Protein	NCBI ID	SENTENCE INDEX