PMID: 6833285

 

    Legend: Gene, Sites

Title : Characterization of the B-chain of human plasma alpha 2HS-glycoprotein

Abstract :
  1. The complete amino acid sequence and primary structure of its heteroglycan
  2. alpha 2HS-Glycoprotein , a normal human plasma protein , was recently shown to consist of two polypeptide chains
  3. In the present study, we have separated these two chains from one another and have elucidated the complete primary structure of the B-chain
  4. Employing automated Edman degradation, the polypeptide moiety of this chain was shown to consist of 27 amino acid residues with an unequal distribution of the neutral and charged amino acid residues
  5. The first 20 residues are uncharged, whereas the carboxyl-terminal heptapeptide contains all charged residues
  6. Utilizing 500-MHz 1H-NMR spectroscopy, the carbohydrate unit proved to be a trisaccharide consisting of sialic acid, galactose, and N-acetylgalactosamine O-glycosidically linked to serine ( residue 6 )
  7. The structure of the B-chain was found to be as follows
  8. (formula; see text) Thus, the molecular weight of the B-chain is 3386
  9. Evaluation of the polypeptide chain by the procedure of Chou and Fasman (Chou, P.Y., and Fasman, G.D. (1979) Adv. Enzymol
  10. 47, 45-148) predicts that the B-chain has two beta-turns
  11. Thereby, the carbohydrate unit which is linked to the Ser residue located in the first beta-turn appears to be directed away from the protein
  12. The second beta-turn probably includes the Cys residue which links the B- to the A-chain
  13. In agreement with the CD analysis, the B-chain lacks beta-conformation but possesses a short alpha-helical region
Output (sent_index, trigger, protein, sugar, site):
  • 0. 2HS-glycoprotein, , alpha 2HS-glycoprotein, -, -
  • 11. linked, , -, the carbohydrate unit, Ser residue
  • 5. contains, , -, all charged residues, heptapeptide
  • 6. linked, , -, a trisaccharide, residue 6
  • 6. linked, , -, a trisaccharide, serine
  • 6. linked, , -, the carbohydrate unit, residue 6
  • 6. linked, , -, the carbohydrate unit, serine
Output(Part-Of) (sent_index, protein, site):
  • 13. B-chain, region
*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

 

Protein NCBI ID SENTENCE INDEX
alpha 2HS-Glycoprotein 197 2
alpha 2HS-glycoprotein 197 0