PMID: 6966283

 

    Legend: Gene, Sites

Title : Studies on the oligosaccharide chains of human alpha 1-protease inhibitor

Abstract :
  1. II
  2. Structure of oligosaccharides
  3. Human alpha 1-protease inhibitor has three oligosaccharide side chains attached to 3 separate asparaginyl residues of the protein by N-glycosyl linkages
  4. Two of the three asparaginyl residues link mostly to the A-type oligosaccharide chains which consist of Man3, Gal2, (GlcNAc)4 and (NeuAc)2
  5. One of the three asparaginyl residues also attaches to a B-type oligosaccharide chain, which consists of Man3, Gal3, (GlcNAc)5, and (NeuAc)3
  6. The ratio of A-chain to B-chain in this particular position is about 2:1
  7. The structures of A- and B-chains of the glycoprotein were examined by periodate oxidation, sequential glycosidase digestion, and permethylation
  8. The results unequivocally revealed the following structure for A-chains
  9. (Formula: see text)
  10. B-type oligosaccharide chains have an additional trisaccharide, i.e. NeuAc 2 alpha leads to 3 Gal 1 beta leads to 4 GlcNAc attached to mannose at the (a) and/or (b) position of A-type chains by a beta 1 leads to 4 linkage
  11. The sialic acid of human alpha 1-protease inhibitor was determined to be N-acetylneuraminic acid
Output (sent_index, trigger, protein, sugar, site):
  • 0. alpha, , alpha 1-protease inhibitor, the oligosaccharide chains, -
  • 10. position, , -, mannose, position
  • 11. inhibitor, , alpha 1-protease inhibitor, N-acetylneuraminic acid, -
  • 11. inhibitor, , alpha 1-protease inhibitor, The sialic acid, -
  • 3. attached, , protein, three oligosaccharide side chains, residues
  • 3. has, , Human alpha 1-protease inhibitor, three oligosaccharide side chains, -
  • 7. glycoprotein, , glycoprotein, -, -
Output(Part-Of) (sent_index, protein, site):
  • 3. protein, residues
*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

 

Protein NCBI ID SENTENCE INDEX
alpha 1-protease inhibitor 5265 0,11
Human alpha 1-protease inhibitor 5265 3