Title : The amino acid
sequence of the
gamma-subunit of mouse submaxillary gland 7 S nerve growth
factor
Abstract :
- The complete amino acid sequence of the gamma-subunit of mouse submaxillary gland 7 S nerve growth factor has been determined from analyses of the peptides generated by cyanogen bromide, trypsin , and chymo trypsin from the naturally occurring fragments
- All peptides were sequenced automatically in a spinning-cup sequenator using Polybrene to minimize extractive losses by the solvents employed thoughout the degradation cycles
- The gamma-subunit , a serine protease with arginine specificity, contains 233 amino acid residues and shares sequence homology with other proteases of this family
- The five disulfide bonds of the gamma-subunit are a subset of the six disulfides present in bovine trypsin , as judged by the location of the half-cystine residues in the primary structure
- An N-linked carbohydrate side chain is attached to Asn-78 in at least a majority of tee gamma-molecules
Output (sent_index, trigger,
protein,
sugar,
site):
- 5. attached, , -, An N-linked carbohydrate side chain, Asn-78
Output(Part-Of) (sent_index,
protein,
site):
- 0. gamma-subunit, sequence
- 1. gamma-subunit, sequence
- 3. gamma-subunit, residues
- 3. protease, residues
*Output_Site_Fusion* (sent_index,
protein,
sugar,
site):
- 5. -, An N-linked carbohydrate side chain, Asn-78