PMID: 7525874

 

    Legend: Gene, Sites

Title : Carbohydrate structures of beta-trace protein from human cerebrospinal fluid: evidence for "brain-type" N-glycosylation

Abstract :

1. The carbohydrate structures of beta-trace protein from human cerebrospinal fluid have been elucidated
2. This protein carries exclusively N-linked oligosaccharides at two sites ( Asn29 and Asn56 )
3. Enzymatically released N-glycans were studied by com position al and methylation analyses, high-pH anion-exchange chromatography, and liquid secondary ion mass spectrometry
4. All glycans were found to be of the complex type, and most (90%) of them were biantennary with no (40%), one (40%), or two (20%) N-acetylneuraminic acid residues
5. The rest were triantennary chains or biantennary chains with intact or truncated lactosamine repeats
6. The innermost N-acetylglucosamine residues of nearly all structures were found to be alpha 1,6-fucosylated
7. Peripheral fucose (about 20% alpha 1,3-linked to N-acetylglucosamine) was also detected
8. Seventy percent of the oligosaccharides contained a bisecting N-acetylglucosamine
9. Especially in the neutral, but also in the monosialylated oligosaccharide fractions, many incomplete antennae consisting of N-acetylglucosamine only were present
10. At least 20 different N-glycans were identified
11. Analysis of the site-specific glycosylation patterns at Asn29 and Asn56 revealed only minor differences
12. According to the structural features (a high degree of fucosylation, high amounts of bisecting N-acetylglucosamine, as well as terminal N-acetylglucosamine and galactose residues , and significant amounts of N-acetylneuraminic acid in alpha 2,3 linkage), this protein can be classified as "brain-type" glycosylated

Output (sent_index, trigger, protein, sugar, site):

• 0. protein, , beta-trace protein, Carbohydrate structures, -
• 1. protein, , beta-trace protein, The carbohydrate structures, -
• 11. glycosylation, , -, -, Asn29 and Asn56
• 12. glycosylated, , protein can, -, -
• 2. carries, , protein, exclusively N-linked oligosaccharides, Asn29 and Asn56
• 2. carries, , protein, exclusively N-linked oligosaccharides, sites
• 6. 1,6-fucosylated, , -, The innermost N-acetylglucosamine residues, -
• 6. 1,6-fucosylated, , -, nearly all structures, -
• 9. monosialylated, , -, the monosialylated oligosaccharide fractions, -

Output(Part-Of) (sent_index, protein, site):

*Output_Site_Fusion* (sent_index, protein, sugar, site):

• 11. beta-trace protein, -, Asn29 and Asn56
• 2. protein, exclusively N-linked oligosaccharides, Asn29 and Asn56

 

Protein	NCBI ID	SENTENCE INDEX
beta-trace protein	5730	0,1