Title : Structures of the N-linked oligosaccharides on human plasma
vitronectin
Abstract :
- The structures of N-linked oligosaccharides present on human plasma vitronectin were elucidated
- Oligosaccharides were released from the vitronectin by N-glycosidase F digestion and tagged with 2-aminopyridine; the pyridylamino-oligosaccharides were then fractionated by anion-exchange and reverse-phase HPLC
- Ten major pyridylamino-oligosaccharides were isolated
- The linkages and locations of sialic acid residues were determined by desialylation with Salmonella sialidase in combination with acid
- The asialo forms were then analyzed by two-dimensional sugar mapping, component sugar analysis and 400-MHz 1H-NMR spectroscopy
- The major oligosaccharides of human vitronectin were of the diantennary N-acetyllactosamine type, with a lesser amount of the tri- and a small amount of the mono-antennary type, to which 1-3 mol sialic acid residues were linked, mostly through alpha 2-6 linkages, although alpha 2-3 linkages were also present
- The possibility that several binding activities of vitronectin can be ascribed to its glycan moiety was discussed, based on the specific features of the N-linked oligosaccharides on human vitronectin revealed here
Output (sent_index, trigger,
protein,
sugar,
site):
- 1. present, , vitronectin, N-linked oligosaccharides, -
- 2. released, , vitronectin, Oligosaccharides, -
- 6. vitronectin, , vitronectin, The major oligosaccharides, -
- 7. oligosaccharides, , vitronectin, oligosaccharides, -
Output(Part-Of) (sent_index,
protein,
site):
*Output_Site_Fusion* (sent_index,
protein,
sugar,
site):