PMID: 7620335

 

    Legend: Gene, Sites

Title : Identification of the oligosaccharide structures of human coagulation factor X factor X activation peptide at each glycosylation site

Abstract :

1. Human blood coagulation factor X factor X has two N-linked oligosaccharides at Asn39 and Asn49 residues and two O-linked oligosaccharides at Thr17 and Thr29 residues in the region of the factor X activation peptide (XAP) which is cleaved off during its activation by factor IXa
2. We determined the structure of oligosaccharides in the XAP region of human factor X . Four glycopeptides each containing a glycosylation site were isolated by digestion of XAP with endoproteinase Asp-N followed by reversed-phase HPLC
3. N-linked oligosaccharides released from the glycopeptides by glycoamidase A digestion were derivatized with 2-aminopyridine
4. Pyridylamino(PA)-oligosaccharides were separated by HPLC into neutral and sialyl oligosaccharides using an anion-exchange column
5. Structures of oligosaccharides and their contents at each glycosylation site were determined by a two-dimensional sugar mapping method
6. The contents of the neutral oligosaccharides at Asn39 and Asn49 residues were 32.5% and 30.0%, respectively
7. Six neutral and twelve monosialyl oligosaccharides isolated from both N-linked glycosylation sites showed similar elution profiles composed of bi-, tri- and tetra-antennary complex type oligosaccharides
8. The predominant component in neutral oligosaccharides was biantennary without a fucose residue
9. Two major monosialyl oligosaccharides were also biantennary without fucose and with a Neu5Ac alpha 2-->6 residue
10. In addition, the structures of O-linked oligosaccharides at Thr17 and Thr29 residues were suggested to be disialylated Gal beta 3GalNAc sequences by their component analyses

Output (sent_index, trigger, protein, sugar, site):

• 0. glycosylation, , -, -, site
• 0. peptide, , -, the oligosaccharide structures, peptide
• 0. site, , -, the oligosaccharide structures, site
• 0. structures, , -, each glycosylation site, site
• 1. has, , factor X, two N-linked oligosaccharides, -
• 1. has, , factor X, two O-linked oligosaccharides, -
• 1. residues, , -, two N-linked oligosaccharides, Asn39 and Asn49 residues
• 1. residues, , -, two N-linked oligosaccharides, Thr17 and Thr29 residues
• 1. residues, , -, two O-linked oligosaccharides, Asn39 and Asn49 residues
• 1. residues, , -, two O-linked oligosaccharides, Thr17 and Thr29 residues
• 10. disialylated, , -, disialylated Gal beta 3GalNAc, -
• 10. residues, , -, disialylated Gal beta 3GalNAc sequences, Thr17 and Thr29 residues
• 2. glycopeptides, , -, -, glycopeptides
• 2. glycosylation, , -, -, site
• 3. glycopeptides, , -, -, glycopeptides
• 3. released, , -, N-linked oligosaccharides, glycopeptides
• 5. glycosylation, , -, -, site
• 6. Asn39, , -, the neutral oligosaccharides, Asn39 and Asn49 residues
• 6. Asn49, , -, the neutral oligosaccharides, Asn39 and Asn49 residues
• 7. glycosylation, , -, -, sites
• 7. isolated, , -, Six neutral and twelve monosialyl oligosaccharides, sites

Output(Part-Of) (sent_index, protein, site):

• 0. coagulation factor X, peptide
• 0. factor X, peptide
• 1. factor X, peptide
• 2. factor X, glycopeptides
• 2. factor X, region

*Output_Site_Fusion* (sent_index, protein, sugar, site):

• 1. factor X, two N-linked oligosaccharides, Asn39 and Asn49 residues
• 1. factor X, two N-linked oligosaccharides, Thr17 and Thr29 residues
• 1. factor X, two O-linked oligosaccharides, Asn39 and Asn49 residues
• 1. factor X, two O-linked oligosaccharides, Thr17 and Thr29 residues
• 10. factor X, disialylated Gal beta 3GalNAc sequences, Thr17 and Thr29 residues
• 6. factor X, the neutral oligosaccharides, Asn39 and Asn49 residues

 

Protein	NCBI ID	SENTENCE INDEX