PMID: 7654718

 

    Legend: Gene, Sites

Title : Isolation and characterization of human milk bile salt-activated lipase C-tail C-tail fragment

Abstract :

1. Glycosylation positions and oligosaccharide characteristics in the proline-rich, mucin-like, C-terminal region ( C-tail ) of human milk bile salt-activated lipase ( BAL ) were studied in order to assess the possible physiological functions of this region
2. A large-scale purification method has been devised to purify the C-tail fragment from human milk BAL
3. Chymotryptic, tryptic, and cyanogen bromide cleavages of partially purified BAL and subsequent molecular sieve chromatography yielded 20-30 mg of C-tail fragment from 1 L of human milk
4. The N-terminal sequence and amino acid com position of the purified C-tail fragment establish that it is derived from residues 528-712 of the enzyme
5. The O-glycosylated carbohydrates of the C-tail fragment contain fucose, galactose, glucosamine, galactosamine, and neuraminic acid in a molar ratio of 1:3: 2:1 :0.3, respectively
6. beta-Elimination reaction revealed that nine threonine residues and less than one serine residue were glycosylated
7. Edman degradation of C-tail fragment and its pronase subfragment suggest a number of glycosylation sites which are flanked by a consensus motif of PVPP
8. We suggest that this motif may serve as a signal for O-glycosylation in the C-tail region of BAL
9. Immunochemical studies indicated that the oligosaccharide chains in the C-tail region of BAL contain Lewis x and Lewis a antigens and, less prominently, sialyl Lewis x and sialyl Lewis a antigens
10. C-tail fragment was also found to bind jacalin lectin
11. These observations suggest the possibility that the C-tail region may contribute to adhesive activity in the physiological function of BAL

Output (sent_index, trigger, protein, sugar, site):

• 1. characteristics, , -, C-terminal region, region
• 1. characteristics, , C-tail, characteristics, -
• 5. O-glycosylated, , -, The O-glycosylated carbohydrates, -
• 5. fragment, , -, The O-glycosylated carbohydrates, fragment
• 6. glycosylated, , -, -, serine residue
• 6. glycosylated, , -, -, threonine residues
• 7. glycosylation, , -, -, sites
• 9. chains, , -, the C-tail region, region

Output(Part-Of) (sent_index, protein, site):

• 0. bile salt-activated lipase C-tail, fragment
• 1. C-tail, positions
• 10. C-tail, fragment
• 11. C-tail, region
• 2. BAL, fragment
• 2. C-tail, fragment
• 3. C-tail, fragment
• 4. C-tail, fragment
• 4. enzyme, residues 528-712
• 5. C-tail, fragment
• 7. C-tail, fragment
• 8. BAL, region
• 8. C-tail, region
• 9. BAL, region
• 9. C-tail, region

*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

Protein	NCBI ID	SENTENCE INDEX
bile salt-activated lipase	1056	1
bile salt-activated lipase C-tail	1056	0