PMID: 7681597

 

    Legend: Gene, Sites

Title : Epitopic structure of Tn glycophorin A for an anti-Tn antibody (MLS 128)

Abstract :
  1. Glycophorin A was digested with glycoprotease (Pasteurella haemolytica) and the digest was fractionated by a combination of high-pressure column chromatographies to produce the glycopeptides GPA-1 to GPA-6
  2. Sequence analysis of the glycopeptides revealed that two serine residues ( Ser-14 and Ser-15 ) are not glycosylated, Thr-17 and Ser-19 being glycosylated instead, in disagreement with the accepted structure
  3. The glycopeptides thus obtained were treated with sialidase and beta-galactosidase
  4. The Tn antigenicity, as assayed by the binding to a monoclonal anti-Tn antibody (MLS 128), was found exclusively in the glycopeptides including three (cluster I) or four (cluster II) consecutive residues of GalNAc-Ser/Thr, whereas the glycopeptide ( GPA-2 ) containing two nonconsecutive GalNAc-Ser/Thr residues had practically no Tn antigenicity
  5. The immunoreactivities of GPA-1 and GPA-3, containing both clusters I and II, and GPA-4, containing cluster II, were 63% (calcd
  6. 67%), 81% (calcd
  7. 86%), and 50% (calcd
  8. 50%), respectively, of the immunoreactivity of GPA-5 or GPA-6, containing cluster I (the average being taken as the basis), based on the reactivity per GalNAc residue
  9. These results indicate that clusters I and II react with the antibody to the same extent
  10. The structure consisting of three consecutive glycosylated Ser/Thr residues may be essential for Tn antigenicity in the light of previous results for ovine submaxillary mucin
Output (sent_index, trigger, protein, sugar, site):
  • 1. glycopeptides, , -, -, glycopeptides
  • 10. glycosylated, , -, -, residues
  • 2. glycopeptides, , -, -, glycopeptides
  • 2. glycosylated, , -, -, Ser-14 and Ser-15
  • 2. glycosylated, , -, -, Thr-17 and Ser-19
  • 2. glycosylated, , -, -, serine residues
  • 2. residues, , -, -, Ser-14 and Ser-15
  • 2. residues, , -, -, serine residues
  • 3. glycopeptides, , -, -, glycopeptides
  • 4. containing, , GPA, two nonconsecutive GalNAc-Ser/Thr residues, glycopeptide
  • 4. glycopeptide, , -, -, glycopeptide
  • 4. glycopeptides, , -, -, glycopeptides
  • 4. three, , -, -, residues
Output(Part-Of) (sent_index, protein, site):
  • 1. GPA, glycopeptides
*Output_Site_Fusion* (sent_index, protein, sugar, site):
  • 2. Tn glycophorin A, -, Ser-14 and Ser-15
  • 2. Tn glycophorin A, -, Thr-17 and Ser-19

 

 

Protein NCBI ID SENTENCE INDEX