PMID: 7727388

 

    Legend: Gene, Sites

Title : The disulfide linkages and glycosylation sites of the human natriuretic peptide receptor-C homodimer

Abstract :

1. The natriuretic peptide receptor-C ( NPR-C ) constitutes greater than 95% of the natriuretic peptide binding sites in vivo
2. This cell surface glycoprotein is a disulfide-linked homodimer with a subunit molecular weight of 68,000
3. Two sources and types of ANP affinity-purified human NPR-C were used to map disulfide linkages and glycosylation sites of this receptor by mass spectrometry: the extracellular domain obtained by papain cleavage of a receptor- IgG fusion protein expressed in Chinese hamster ovary cells, and a baculovirus/Sf9-expressed cytoplasmic truncation mutant in which 34 of 37 cytoplasmic domain amino acids were deleted
4. Two intramolecular disulfide bonded loops were found in the 435 amino acid extracellular domain (C63-C91, C168-C216)
5. The juxtamembrane residues C428 and C431 are involved in homodimer formation, confirmed by site-directed mutagenesis of full-length NPR
6. Three of the four potential Asn-linked glycosylation sites are occupied: N41 (complex), N248 (high mannose), and N349 (complex; partial occupancy)
7. These data describe the intra- and intermolecular linkages in NPR-C , providing a model for the homologous guanylyl cyclase receptors , NPR-A and NPR-B ; both of the cyclase receptors likely contain the first amino-terminal 29 amino acid loop, but only NPR-A possesses the second 49 amino acid loop in common with NPR-C

Output (sent_index, trigger, protein, sugar, site):

• 0. glycosylation, , natriuretic peptide receptor-C homodimer, -, sites
• 2. glycoprotein, , glycoprotein, -, -
• 2. glycoprotein, , homodimer, -, -
• 3. glycosylation, , receptor, -, sites
• 6. glycosylation, , -, -, sites
• 6. occupied, , -, -, N248
• 6. occupied, , -, -, N349
• 6. occupied, , -, -, N41
• 6. occupied, , -, -, sites

Output(Part-Of) (sent_index, protein, site):

• 0. natriuretic peptide receptor-C homodimer, sites
• 3. receptor, sites

*Output_Site_Fusion* (sent_index, protein, sugar, site):

• 6. natriuretic peptide receptor-C homodimer, -, N248
• 6. natriuretic peptide receptor-C homodimer, -, N349
• 6. natriuretic peptide receptor-C homodimer, -, N41

 

Protein	NCBI ID	SENTENCE INDEX
NPR-A	4881	7
homodimer	6647	2,5
natriuretic peptide receptor-C homodimer	4883	0
NPR-B	4882	7
NPR	23467	5