Title : Identification and structural characterization of a mannose-6-phosphate containing oligomannosidic N-glycan from human
erythropoietin secreted by recombinant BHK-21 cells
Abstract :
- A sialidase resistant mono-charged N-glycan was isolated from glycosylation site I ( Asn-24 ) of recombinant human erythropoietin expressed from baby hamster kidney (BHK-21) cells and constituted approximately 2-4% of the oligosaccharide material at this glycosylation site
- Mass spectrometry and both 1- and 2-dimensional NMR techniques revealed a high mannose type structure (Man6) with a phospho-diesterbridged N-acetylglucosamine as follows: [formula: see text
Output (sent_index, trigger,
protein,
sugar,
site):
- 0. erythropoietin, , erythropoietin, a mannose-6-phosphate containing oligomannosidic N-glycan, -
- 1. glycosylation, , -, -, site
- 1. glycosylation, , erythropoietin, -, Asn-24
- 1. glycosylation, , erythropoietin, -, site
Output(Part-Of) (sent_index,
protein,
site):
- 1. erythropoietin, Asn-24
- 1. erythropoietin, site
*Output_Site_Fusion* (sent_index,
protein,
sugar,
site):
- 1. erythropoietin, -, Asn-24