Title : Molecular cloning and sequence analysis of the monkey and human
tissue kallikrein genes
Abstract :
- Cynomolgus monkey renal kallikrein cDNA and genomic human tissue kallikrein gene were cloned
- The monkey gene encodes a 257 amino acid (aa) preprokallikrein and exhibits 95% and 92% homology to the human at nucleotide (nt) and aa level, respectively
- The monkey gene encodes a 233-aa mature kallikrein versus a 238-aa in human
- The human kallikrein gene and urinary kallikrein both contain a Lys-162 instead of the reported Glu-162
- Human, monkey and rat renal/pancreatic kallikrein genes evolve with a N-glycosylation containing domain (aa 81-87) which is absent in porcine and is non-glycosylable in mice
- Only human kallikrein evolves with an additional Thr-108 and with a N-glycosylation site at aa-141
Output (sent_index, trigger,
protein,
sugar,
site):
- 6. N-glycosylation, , -, -, site
Output(Part-Of) (sent_index,
protein,
site):
*Output_Site_Fusion* (sent_index,
protein,
sugar,
site):