PMID: 8223648

 

    Legend: Gene, Sites

Title : Expression of human interferon omega 1 in Sf9 cells

Abstract :
  1. No evidence for complex-type N-linked glycosylation or sialylation
  2. Human interferon omega 1 ( IFN-omega 1 ) was expressed in Spodoptera frugiperda Sf9 insect cells using the baculovirus expression system
  3. Half of the protein purified by immunoaffinity chromatography was shown to be N-glycosylated at the same site as the natural IFN-omega 1
  4. The degree of glycosylation was independent of the expression rate
  5. While natural IFN-omega 1 was shown to carry complex-type oligosaccharides [Adolf, G. R., Maurer-Fogy, I., Kalsner, I. & Cantell, K. (1990) J. Biol
  6. Chem
  7. 265, 9290-9295], the insect cell produced protein which was demonstrated by lectin blot, mass spectroscopy and HPLC analysis to contain only the core oligosaccharide
  8. Two different structures, (Man)2(GlcNAc)2[Fuc] and (Man)3(GlcNAc)2[Fuc] were identified
  9. The fucosylation was identified to be ( alpha 1-6 )-linked to the core saccharide
  10. Sialic acid residues were clearly absent
  11. IFN-omega 1 expressed in S. frugiperda cells was shown to be partially truncated at the C-terminus by nine residues; its antiviral activity when glycosylated was significantly lower than the activity of IFN-omega 1 produced by Sendai-virus-stimulated leukocytes
  12. Circular dichroism and fluorescence spectroscopy did not reveal any structural differences between glycosylated and nonglycosylated IFN-omega 1
  13. This implies the importance of a complex-type glycosylation for the maximal biological activity of human IFN-omega 1
Output (sent_index, trigger, protein, sugar, site):
  • 12. glycosylated, , IFN-omega 1, -, -
  • 12. nonglycosylated, , IFN-omega 1, -, -
  • 3. N-glycosylated, , Half, -, site
  • 5. carry, , IFN-omega 1, complex-type oligosaccharides, -
  • 7. contain, , protein, only the core oligosaccharide, -
Output(Part-Of) (sent_index, protein, site):
*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

 

Protein NCBI ID SENTENCE INDEX
interferon omega 1 3467 0
IFN-omega 1 3467 2,3,5,11,12,13
Human interferon omega 1 3467 2
alpha 1-6 146 9