PMID: 8329384

 

    Legend: Gene, Sites

Title : Identification and structural analysis of the tetrasaccharide NeuAc alpha(2-->6)Gal beta(1-->4)GlcNAc beta(1-->3)Fuc alpha 1-->O-linked to serine 61 of human factor IX

Abstract :

1. O-Linked fucose has been found attached to Thr/Ser residues within the sequence Cys-X-X-Gly-Gly-Thr/Ser- Cys in the N-terminal EGF domains of several coagulation/fibrinolytic proteins
2. Carbohydrate composition and mass spectrometric analyses of tryptic and thermolytic peptides containing the corresponding site ( Ser-61 ) in the first EGF domain of human factor IX indicated the presence of a tetrasaccharide containing one residue each of sialic acid, galactose, N-acetylglucosamine, and fucose
3. The Ser-61 tetrasaccharide was not susceptible to alpha-fucosidase digestion
4. Fragments generated during mass spectrometric analysis indicated that fucose was the attachment sugar residue
5. The involvement of fucose in the carbohydrate-peptide linkage was confirmed by two-dimensional 1H NMR spectroscopic analysis of the glycopeptide containing factor IX residues 57-65
6. The complete structure of the tetrasaccharide was obtained by methylation analysis and two-dimensional 1H TOCSY and ROESY experiments as NeuAc alpha(2-->6)Gal beta(1-->4)GlcNAc beta(1-->3)Fuc alpha 1-->O- Ser61

Output (sent_index, trigger, protein, sugar, site):

• 1. attached, , -, O-Linked fucose, residues
• 2. peptides, , -, Carbohydrate composition, peptides
• 5. IX, , -, -, residues 57-65
• 5. glycopeptide, , -, -, glycopeptide

Output(Part-Of) (sent_index, protein, site):

• 0. factor IX, serine 61
• 1. N-terminal EGF, domains
• 1. proteins, Cys
• 1. proteins, domains
• 2. -, Ser-61
• 2. -, site
• 2. EGF, domain
• 2. factor IX, domain

*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

Protein	NCBI ID	SENTENCE INDEX