PMID: 8336134

 

    Legend: Gene, Sites

Title : Purification and chemical characterization of beta-trace protein from human cerebrospinal fluid: its identification as prostaglandin D synthase

Abstract :

1. beta-Trace protein from pooled human CSF was purified to homogeneity
2. An apparent molecular mass of 23-29 kDa was determined for the polypeptide on sodium dodecyl sulfate-polyacrylamide gel electrophoresis
3. Amino-terminal sequencing of the polypeptide yielded the unique amino acid sequence APEAQVSVQPNFQQDKFLGRWFSA
4. Alignment of amino acid sequences obtained from tryptic peptides with the sequence previously deduced from a cDNA clone isolated by other investigators allowed the identification of beta-trace protein as prostaglandin D synthase [ prostaglandin-H2 D-isomerase ; (5Z, 13E)-(15S)-9 alpha, 11 alpha-epidioxy-15-hydroxyprosta-5,13-dienoate D-isomerase ; EC 5.3.99.2]
5. A conservative amino acid exchange ( Thr instead of Ser ) was detected at amino acid position 154 of the beta-trace polypeptide chain in the corresponding tryptic peptide
6. The two N-glycosylation sites of the polypeptide were shown to be almost quantitatively occupied by carbohydrate
7. Carbohydrate compositional as well as methylation analysis indicated that Asn29 and Asn56 bear exclusively complex-type oligosaccharide structures (partially sialylated with alpha 2-3- and/or alpha 2-6-linked N-acetylneuraminic acid) that are almost quantitatively alpha 1-6 fucosylated at the proximal N-acetylglucosamine; approximately 70% of these molecules contain a bisecting N-acetylglucosamine
8. Agalacto structures as well as those with a peripheral fucose are also present

Output (sent_index, trigger, protein, sugar, site):

• 6. N-glycosylation, , -, -, polypeptide
• 6. N-glycosylation, , -, -, sites
• 6. occupied, , -, -, polypeptide
• 6. occupied, , -, -, sites
• 7. bear, , -, complex-type oligosaccharide structures, Asn29 and Asn56
• 7. fucosylated, , -, complex-type oligosaccharide structures, -
• 7. sialylated, , -, complex-type oligosaccharide structures, -

Output(Part-Of) (sent_index, protein, site):

• 4. -, sequence

*Output_Site_Fusion* (sent_index, protein, sugar, site):

• 7. prostaglandin D synthase, complex-type oligosaccharide structures, Asn29 and Asn56

 

Protein	NCBI ID	SENTENCE INDEX
prostaglandin-H2 D-isomerase	5730	4
beta-Trace protein	5730	1
CSF	1437	1
prostaglandin D synthase	5730	0,4
beta-trace protein	5730	0,4