PMID: 8344278

 

    Legend: Gene, Sites

Title : Structural study of the oligosaccharide moieties of sphingolipid activator proteins , saposins A, C and D obtained from the spleen of a Gaucher patient

Abstract :

1. We have determined and compared the structures of the oligosaccharide moieties of saposin A , C and D purified from the spleen of a patient with Gaucher disease
2. These saposins, together with saposin B , are small glycoproteins , derived from separate domains of a single precursor , prosaposin , and are required for the lysosomal hydrolysis of various sphingolipids
3. The characteristic features of the oligosaccharide moieties of saposin A are (a) the predominance of a fucosylated trimannosyl core structure and (b) the occurrence of several different oligomannose-type and N-acetyllactosamine-type oligosaccharides
4. Saposin C contains (a) a predominance of oligomannose-type oligosaccharides and monoantennary oligosaccharides and (b) the presence of four different oligosaccharides having bisecting N-acetylglucosamine residues (found only in this saposin )
5. Saposin D is distinguished by the occurrence of oligomannose-type oligosaccharides, which comprise nearly 90% of its total oligosaccharides
6. The possible reasons for the unique glycosylation of each saposin is discussed

Output (sent_index, trigger, protein, sugar, site):

• 0. proteins, , proteins, the oligosaccharide moieties, -
• 1. A, , saposin A, the oligosaccharide moieties, -
• 2. glycoproteins, , glycoproteins, -, -
• 3. A, , saposin A, the oligosaccharide moieties, -
• 3. fucosylated, , -, a fucosylated trimannosyl core structure, -
• 4. found, , saposin, bisecting N-acetylglucosamine residues, -
• 6. glycosylation, , saposin, -, -

Output(Part-Of) (sent_index, protein, site):

• 2. precursor, domains

*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

Protein	NCBI ID	SENTENCE INDEX