Title : An improved approach to the analysis of the structure of small oligosaccharides of
glycoproteins : application to the O-linked oligosaccharides from human
glycophorin A . Treatment of purified human
glycophorin A with alkaline borohydride cleaved the oligosaccharide side chains to yield alditol derivatives that were separated by gel filtration into three mixtures of low molecular weight compounds
Abstract :
- Each mixture was oxidised with periodate, and the products were reduced with borohydride and analysed after acetylation or methylation by GLC-MS and FABMS
- The resulting data allowed the monosaccharide sequence and linkage positions to be assigned to each component of the mixtures
- The anomeric configuration was determined by 1H NMR spectroscopy of the intact fractions
- The structures of a desialylated tetrasaccharide, two monosialylated trisaccharides, and five other minor products were defined
Output (sent_index, trigger,
protein,
sugar,
site):
- 0. glycoproteins, , glycoproteins, small oligosaccharides, -
- 4. desialylated, , -, a desialylated tetrasaccharide, -
- 4. monosialylated, , -, two monosialylated trisaccharides, -
Output(Part-Of) (sent_index,
protein,
site):
*Output_Site_Fusion* (sent_index,
protein,
sugar,
site):