PMID: 8404899

 

    Legend: Gene, Sites

Title : Variation of the glycosylation of human pancreatic bile-salt-dependent lipase

Abstract :

1. Glycoproteins of human pancreatic juice were characterized by means of lectins after electrophoresis and electrotransfer to nitrocellulose membranes
2. For the detected glycoproteins , only a 100-kDa glycoprotein varied in the pancreatic juice from a normal patient (i.e. without any pancreatic disorder) compared to the pancreatic juice from a patient suffering from chronic pancreatitis
3. This protein , which is the only protein in human pancreatic juice which is O-glycosylated and N-glycosylated, was identified as the bile-salt-dependent lipase
4. Among the glycosylated proteins present in human pancreatic juice, only the glycosylation of bile-salt-dependent lipase differs between individuals
5. The enzyme was isolated either from normal or pathological human pancreatic juices
6. The purified variants have an identical molecular mass and amino-acid com position
7. As suspected from lectin affinity studies, the oligosaccharide composition differs between the variants
8. The structure of the N-linked oligosaccharides of the variant from the pancreatic juice of a normal donor correlated with complete processing and maturation of a complex-type N-glycan
9. Alteration of the maturation process can be detected for a bile-salt-dependent- lipase variant from a patient suffering with chronic pancreatitis, since the carbohydrate composition is compatible with the predominance of hybrid or high-mannose-type structures
10. The amount of sugar involved in O-glycosylation associated with the peanut agglutinin reactivity suggests the presence of 12-14 minimal Gal beta 1-->3GalNac-->T/S O-glycan structures which are sialylated and fucosylated
11. The amount of sugar involved in the O-linked oligosaccharide structure appears to be unchanged in the variants isolated from the pathological pancreatic juice

Output (sent_index, trigger, protein, sugar, site):

• 0. glycosylation, , lipase, -, -
• 10. sialylated, , -, 12-14 minimal Gal beta 1-->3GalNac-->T/S O-glycan structures, -
• 11. variants, , variants, sugar, -
• 2. glycoprotein, , glycoprotein, -, -
• 2. glycoproteins, , glycoproteins, -, -
• 3. O-glycosylated, , protein, -, -
• 4. glycosylated, , proteins, -, -
• 4. glycosylation, , lipase, -, -
• 8. variant, , variant, the N-linked oligosaccharides, -

Output(Part-Of) (sent_index, protein, site):

• 6. variants, position

*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

Protein	NCBI ID	SENTENCE INDEX