Title : Isolation and structural characterization of novel sialylated oligosaccharide-alditols from respiratory-mucus
glycoproteins of a patient suffering from bronchiectasis
Abstract :
- The carbohydrate chains of the respiratory-mucus glycoproteins of a patient (blood group O) suffering from bronchiectasis due to Kartagener's syndrome, were released by alkaline borohydride treatment of a pronase digest
- The structures of 82 neutral and low-molecular-mass sialylated oligosaccharides have been described previously [van Kuik A., de Waard P., Vliegenthart J. F. G., Klein A., Carnoy C., Lamblin G . Roussel P. (1991) Eur
- J. Biochem
- 198, 169-182]
- In the present work, medium-size sialylated oligosaccharides were obtained after ion-exchange chromatography and were subsequently separated into 36 fractions utilizing gel filtration, HPLC on normal-phase alkylamine-bonded silica and reverse-phase HPLC
- From these fractions, the following six sialylated hepta- and octa-saccharide-alditols have been characterized by employing 500-MHz 1H-NMR spectroscopy, in conjunction with fast-atom-bombardment mass spectroscopy and methylation analysis
- [formula: see text
Output (sent_index, trigger,
protein,
sugar,
site):
- 0. glycoproteins, , glycoproteins, -, -
- 0. sialylated, , -, novel sialylated oligosaccharide-alditols, -
- 1. glycoproteins, , glycoproteins, The carbohydrate chains, -
- 2. sialylated, , -, sialylated oligosaccharides, -
- 5. sialylated, , -, medium-size sialylated oligosaccharides, -
Output(Part-Of) (sent_index,
protein,
site):
*Output_Site_Fusion* (sent_index,
protein,
sugar,
site):