PMID: 8486673

 

    Legend: Gene, Sites

Title : Studies on the carbohydrate moiety and on the biosynthesis of rat C-reactive protein

Abstract :
  1. Rat C-reactive protein ( CRP ) is a pentameric glycoprotein composed of five apparently identical monomers, two of which form a disulfide-linked dimer (Rasosouli, M., Sambasivam, H., Azadi, P., Dell, A., Morris, H. R., Nagpurkar, A., Mookerjea, S., and Murray, R. K. (1992) J. Biol
  2. Chem
  3. 267, 2947-2954)
  4. In this study, the nature of the oligosaccharide chain of rat CRP was investigated by fast atom bombardment-mass spectrometry (FAB-MS), and general features of its biosynthetic pathway were also analyzed
  5. FAB-MS, electrospray-mass spectrometry, and linkage analysis demonstrated that each monomer of rat CRP contained one oligosaccharide chain, predominantly a disialylated biantennary structure, attached to Asn-128
  6. The biosynthesis of rat CRP was studied by immunoprecipitation of CRP synthesized in vitro and by cultured hepatocytes
  7. The results revealed that each monomer of rat CRP was synthesized individually as a single-chain precursor with a cleavable signal sequence
  8. The translocated species was sensitive to digestion by endoglycosidase H (endo H), indicating that it possessed a high mannose oligosaccharide
  9. Rat CRP acquired the ability to bind to phosphorylcholine-Sepharose and to form the dimeric and oligomeric species prior to acquiring resistance to endo H. Studies using tunicamycin revealed that the N-linked oligosaccharide present in rat CRP was not required for formation of its dimeric component, oligomerization, ability to bind to phosphorylcholine, or secretion
  10. The non-glycosylated rat CRP , however, was still able to bind to phosphorylcholine-Sepharose and to be secreted by hepatocytes
Output (sent_index, trigger, protein, sugar, site):
  • 1. glycoprotein, , Rat C-reactive protein, -, -
  • 1. glycoprotein, , glycoprotein, -, -
  • 10. non-glycosylated, , CRP, -, -
  • 4. CRP, , CRP, the oligosaccharide chain, -
  • 5. attached, , -, one oligosaccharide chain, Asn-128
  • 5. contained, , CRP, one oligosaccharide chain, -
  • 5. disialylated, , -, a disialylated biantennary structure, -
Output(Part-Of) (sent_index, protein, site):
  • 7. precursor, sequence
*Output_Site_Fusion* (sent_index, protein, sugar, site):
  • 5. CRP, one oligosaccharide chain, Asn-128

 

 

Protein NCBI ID SENTENCE INDEX
CRP 25419 1,4,5,6,7,10
Rat CRP 25419 9
C-reactive protein 25419 0
Rat C-reactive protein 25419 1