PMID: 8507662

 

    Legend: Gene, Sites

Title : Pro-major basic protein has three types of sugar chains at the pro-portion

Abstract :

1. The amino-acid sequence of purified recombinant pro-major basic protein from Chinese hamster kidney cells was determined to verify the primary structure and glycosylation sites
2. Reduced and S-carboxamidemethylated protein was first digested with Achromobacter proteinase I. Each peptide was characterized by amino-acid analysis and amino-acid sequence analysis
3. We could identify all the peptides which were expected from the pro-major basic protein cDNA sequence
4. Sequence analysis and deglycosylation study revealed that Ser-8, Thr-9, Ser-46 and Asn-70 were glycosylated
5. The results indicated that proMBP has three types of sugar chains, O-glycoside, N-glycoside and glycosaminoglycan, in the pro-portion

Output (sent_index, trigger, protein, sugar, site):

• 1. glycosylation, , -, -, sites
• 4. glycosylated, , -, -, Ser-8, Thr-9, Ser-46 and Asn-70

Output(Part-Of) (sent_index, protein, site):

• 1. protein, sequence
• 3. cDNA, sequence
• 3. protein, sequence

*Output_Site_Fusion* (sent_index, protein, sugar, site):

• 4. S-carboxamidemethylated, -, Ser-8, Thr-9, Ser-46 and Asn-70

 

Protein	NCBI ID	SENTENCE INDEX