Title : Biological and physicochemical characterization of recombinant human erythropoietins fractionated by Mono Q column chromatography and their modification with
sialyltransferase
Abstract :
- Ten erythropoietin ( EPO ) fractions differing in sialic acid content, ranging from 9.5 to 13.8 mol mol-1 of EPO , were obtained from baby hamster kidney cell-derived recombinant human EPO by Mono Q column chromatography
- The mean pI values of the EPO fractions determined by IEF-gel electrophoresis systematically shifted from 4.11 to 3.31, coinciding with the sialic acid content, without a change in the constitution of asialo N-linked oligosaccharides of each fraction
- Although a linear relationship between the in vivo bioactivity and the sialic acid content of the fractionated samples was observed until 12.1 mol mol-1 of EPO , there was no further increase in their activity over 12.4 mol mol-1 of EPO
- On the other hand, an inverse relationship between the in vitro bioactivity and sialic acid content of EPO was observed
- Also, we showed that the in vivo bioactivity of some fractions with low sialic acid contents was increased after treatment with alpha 2,6-sialyltransferase , but the in vivo bioactivity of the other fractions with high sialic acid contents was either decreased or not affected
Output (sent_index, trigger,
protein,
sugar,
site):
- 4. EPO, , EPO, sialic acid content, -
Output(Part-Of) (sent_index,
protein,
site):
*Output_Site_Fusion* (sent_index,
protein,
sugar,
site):