PMID: 9524075

 

    Legend: Gene, Sites

Title : Sorting of non-glycosylated human procathepsin S in mammalian cells

Abstract :
  1. Cathepsin S , a lysosomal cysteine protease , is synthesized as inactive precursor
  2. It is activated in the lysosomes by a proteolytic cleavage of the propeptide
  3. HEK 293-cells which do not express cathepsin S were transfected with cDNA of either wild type human procathepsin S or a mutant procathepsin S in which Asn of the only glycosylation site in the proregion was replaced by Gln
  4. The cells expressed glycosylated and non-glycosylated procathepsin S , respectively
  5. Large amounts of the precursors were secreted into the culture media by both transfectants
  6. Secreted wild type procathepsin S contained Man-6-phosphate in the oligosaccharide chain
  7. Wild type procathepsin S was activated in the cells but no maturation occurred in the culture media
  8. In vitro processing of glycosylated as well as of non-glycosylated procathepsin S gave fully active enzymes thus indicating that the oligosaccharide chain was not necessary for proper folding
  9. A reuptake of the glycosylated and non-glycosylated procathepsin S by HEK 293-cells could be observed
  10. Small amounts of mature cathepsin S were detected in the lysosomes of the mutant transfectants
  11. Subcellular fractionation showed non-glycosylated procathepsin S in the membrane fraction
  12. Non-glycosylated procathepsin S was bound to the plasma membrane at 2 degrees C, suggesting an additional sorting motif in the cathepsin S molecule besides the Man-6-phosphate residue
Output (sent_index, trigger, protein, sugar, site):
  • 0. non-glycosylated, , procathepsin S, -, -
  • 11. non-glycosylated, , non-glycosylated procathepsin S, -, -
  • 12. Non-glycosylated, , Non-glycosylated procathepsin S, -, -
  • 3. glycosylation, , -, -, site
  • 8. non-glycosylated, , non-glycosylated procathepsin S, -, -
  • 9. glycosylated, , the glycosylated and non-glycosylated procathepsin S, -, -
  • 9. non-glycosylated, , the glycosylated and non-glycosylated procathepsin S, -, -
Output(Part-Of) (sent_index, protein, site):
*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

 

Protein NCBI ID SENTENCE INDEX
cathepsin S 1520 3,10,12
Cathepsin S 1520 1