Title : Effects of glycosylation of the
residue at position 14 in ovine
angiotensinogen on the human
renin reaction
Abstract :
- A mutant angiotensinogen , S14N, in which Ser14 of ovine angiotensinogen was replaced by Asn to form a N-glycosylation site , was produced in CHO cells
- The molecular weight was about 3,000 larger than that of wild-type ovine angiotensinogen , indicating that S14N angiotensinogen was glycosylated at Asn14
- In the reaction with human renin , the km of mutant angiotensinogen was 3 times increased, but the Vmax was not affected by the mutation
Output (sent_index, trigger,
protein,
sugar,
site):
- 0. glycosylation, , -, -, residue at position 14
- 0. glycosylation, , renin, -, residue at position 14
- 1. N-glycosylation, , -, -, site
- 2. glycosylated, , S14N angiotensinogen, -, Asn14
Output(Part-Of) (sent_index,
protein,
site):
- 0. angiotensinogen, residue at position 14
- 1. angiotensinogen, Ser14
*Output_Site_Fusion* (sent_index,
protein,
sugar,
site):
- 2. S14N angiotensinogen, -, Asn14