Title : Isolation and characterization of circulating 13-kDa C-terminal
fragments of human
insulin-like growth
factor binding
protein-5
Abstract :
- The insulin-like growth factor binding proteins ( IGFBPs ) are responsible for regulation of the effects and the bioavailability of the insulin-like growth factors ( IGFs )
- We screened for circulating fragments of human IGFBP-5 in human hemofiltrate
- Identification of IGFBP-5 peptides in the fractions of our peptide bank generated from hemofiltrate was performed by their immunoreactivity and their capacity to bind IGF-I
- Different fragments of IGFBP-5 with molecular sizes from 12 to 25 kDa were identified
- C-terminal peptides of IGFBP-5 with molecular masses of 13.3 and 13.5 kDa were purified by consecutive chromatographic steps and sequenced
- Sequence analysis of the peptides revealed the (double) sequences (K)FVGGAENXAHPRII and MVPRAVYLPNXDRKG
- In addition, a smaller fragment with Mr 2722 of the central IGFBP-5 region was purified and showed the sequence HTRISELKAEAVKKDRRKKLTQS ( residues 121-143 ) indicating plasma proteolysis of IGFBP-5 C-terminal to amino acids Lys-120, Ser-143, Lys-144, and Arg-188
- According to mass spectrometric and sequence analysis, Thr-152 was shown to be O-glycosylated
- Fractions containing C-terminal IGFBP-5 fragments revealed significant IGF-I binding properties
- Our results indicate that plasma proteolysis of IGFBP-5 preferentially occurs C-terminally to basic residues and generates different C-terminal fragments , possibly acting in an IGF-dependent manner and bearing intrinsic biological functions
Output (sent_index, trigger,
protein,
sugar,
site):
- 8. O-glycosylated, , -, -, Thr-152
Output(Part-Of) (sent_index,
protein,
site):
- 0. insulin-like growth factor binding protein-5, fragments
- 2. IGFBP-5, fragments
- 3. IGFBP-5, peptides
- 4. IGFBP-5, fragments
- 5. IGFBP-5, peptides
- 7. IGFBP-5, region
- 9. IGFBP-5, fragments
*Output_Site_Fusion* (sent_index,
protein,
sugar,
site):
- 8. insulin-like growth factor binding protein-5, -, Thr-152