PMID: 9884403

 

    Legend: Gene, Sites

Title : Glycosylation sites and site-specific glycosylation in human Tamm-Horsfall glycoprotein

Abstract :
  1. The N-glycosylation sites of human Tamm-Horsfall glycoprotein from one healthy male donor have been characterized, based on an approach using endoproteinase Glu-C ( V-8 protease , Staphylococcus aureus ) digestion and a combination of chromatographic techniques, automated Edman sequencing, and fast atom bombardment mass spectrometry
  2. Seven out of the eight potential N-glycosylation sites , namely, Asn52, Asn56, Asn208, Asn251, Asn298, Asn372, and Asn489 , turned out to be glycosylated, and the potential glycosylation site at Asn14 , being close to the N-terminus, is not used
  3. The carbohydrate microheterogeneity on three of the glycosylation sites was studied in more detail by high-pH anion-exchange chromatographic profiling and 500 MHz1H-NMR spectroscopy
  4. Glycosylation site Asn489 contains mainly di- and tri-charged oligosaccharides which comprise, among others, the GalNAc4 S (beta1-4)GlcNAc terminal sequence
  5. Only glycosylation site Asn251 bears oligomannose-type carbohydrate chains ranging from Man5GlcNAc2to Man8GlcNAc2, in addition to a small amount of complex-type structures
  6. Profiling of the carbohydrate moieties of Asn208 indicates a large heterogeneity, similar to that established for native human Tamm-Horsfall glycoprotein , namely, multiply charged complex-type carbohydrate structures, terminated by sulfate groups, sialic acid residues, and/or the Sda-determinant
Output (sent_index, trigger, protein, sugar, site):
  • 0. glycoprotein, , Tamm-Horsfall glycoprotein, -, -
  • 0. glycosylation, , Tamm-Horsfall glycoprotein, -, -
  • 1. N-glycosylation, , Tamm-Horsfall glycoprotein, -, sites
  • 1. glycoprotein, , Tamm-Horsfall glycoprotein, -, -
  • 2. N-glycosylation, , -, -, sites
  • 2. glycosylated, , -, -, Asn52, Asn56, Asn208, Asn251, Asn298, Asn372, and Asn489
  • 2. glycosylation, , -, -, Asn14
  • 2. glycosylation, , -, -, site
  • 2. used, , -, -, Asn14
  • 2. used, , -, -, site
  • 3. glycosylation, , -, -, sites
  • 3. microheterogeneity, , -, the glycosylation sites, sites
  • 4. contains, , -, di-, Asn489
  • 4. contains, , -, tri-charged oligosaccharides, Asn489
  • 5. glycosylation, , -, -, site Asn251
  • 6. Asn208, , -, the carbohydrate moieties, Asn208
  • 6. glycoprotein, , Tamm-Horsfall glycoprotein, -, -
Output(Part-Of) (sent_index, protein, site):
  • 0. Tamm-Horsfall glycoprotein, sites
  • 1. Tamm-Horsfall glycoprotein, sites
*Output_Site_Fusion* (sent_index, protein, sugar, site):
  • 2. Tamm-Horsfall glycoprotein, -, Asn14
  • 2. Tamm-Horsfall glycoprotein, -, Asn52, Asn56, Asn208, Asn251, Asn298, Asn372, and Asn489
  • 4. Tamm-Horsfall glycoprotein, di-, Asn489
  • 4. Tamm-Horsfall glycoprotein, tri-charged oligosaccharides, Asn489
  • 5. Tamm-Horsfall glycoprotein, -, site Asn251
  • 6. Tamm-Horsfall glycoprotein, the carbohydrate moieties, Asn208

 

 

Protein NCBI ID SENTENCE INDEX