PMID: PMC2628603-1-1

 

    Legend: Gene, Sites

Title : Erythrocytic proteins are O-GlcNAcylated

Abstract :

1. We initially tested the extent of GlcNAcylation in erythrocytic proteins by using a pan-specific O-GlcNAc antibody (CTD 110.6)
2. Immunoblot data showed that multiple erythrocytic proteins are O-GlcNAc modified (Fig. 1)
3. Control immunoblotting with O-GlcNAc antibody but in the presence of excess free GlcNAc yielded little to no signal, suggesting the specificity of the blotting (data not shown)
4. We next wanted to assess whether the GlcNAcylation cycles in erythrocytes
5. Both OGT and O-GlcNAcase , the enzymes responsible for O-GlcNAc cycling, are detected in erythrocytes (Fig. 1)
6. The donor substrate for OGT , UDP-GlcNAc , and O-GlcNAcase activity are also present in the erythrocytes (K.P., C.S., G.H., unpublished data)
7. These data suggest that O-GlcNAc cycling may exist in human erythrocytes

Output (sent_index, trigger, protein, sugar, site):

Output(Part-Of) (sent_index, protein, site):

*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

Protein	NCBI ID	SENTENCE INDEX