PMID: PMC3617342-1-3

 

    Legend: Gene, Sites

Title : The Secretome of Activated ECs

Abstract :
  1. HUVECs were stimulated with PMA, a commonly used secretagogue that induces exocytosis of endothelial vesicles
  2. As previously reported (26), the morphology of ECs changes from spindle-shaped to round upon PMA activation, and the rod-shaped Weibel-Palade bodies, unique storage vesicles within ECs containing vWF and many other secreted proteins , fuse with the cell membrane (Fig. 1A)
  3. In total, the secretomes of 17 primary ECs were analyzed via gel-LC-MS/MS, with or without deglycosylation
  4. Apart from 123 secreted proteins , the conditioned medium of PMA-stimulated ECs was particularly rich in surface antigens and receptors , including many established endothelial markers (Table I)
  5. All identified proteins and peptides are listed in supplemental Tables S1 and S2, respectively
  6. The distribution of the frequencies and the cumulated distribution of the number of samples in which proteins were identified are shown in supplemental Fig
  7. S1
  8. MS datasets of three biological replicates have been deposited in PRIDE (accession numbers 26908–27003)
  9. Immunoblots confirmed that proteins such as fibronectin and biglycan were constitutively secreted (Fig. 1B)
  10. Others such as agrin and lymphatic vessel endothelial hyaluronic acid receptor 1 were released upon PMA stimulation, providing an explanation for why previously unidentified proteins (8, 10) were found in the present analysis (Fig. 1C)
  11. An overlay between secreted (Cy3 and Cy 5; green and red color) and cellular (Cy 2; blue color) proteins is shown in Fig. 1D
  12. Common spots were numbered (supplemental Fig
  13. S2) and identified via LC-MS/MS (supplemental Table S3)
  14. Certain proteins , such as von Willebrand antigen 2 (a propeptide of vWF , AA 23–763), were clearly more abundant in the secretome of PMA-treated HUVECs
Output (sent_index, trigger, protein, sugar, site):
Output(Part-Of) (sent_index, protein, site):
  • 14. vWF, propeptide
*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

 

Protein NCBI ID SENTENCE INDEX