PMID: PMC3617342-1-5

 

    Legend: Gene, Sites

Title : Validation of Glyco proteins

Abstract :

1. To validate the glycosylation status, we performed additional analysis before and after glycoprotein enrichment with affinity resins of ConA lectin (n = 4) using a Q Exactive MS (Thermo Scientific)
2. We then compared the number of identified spectra in the glycoprotein-enriched fraction, the flow-through, and the input (supplemental Table S5)
3. For most glycoproteins , a higher spectral count was observed in the glycoprotein-enriched fraction than in the original input and/or the flow-through
4. Representative examples ( fibronectin , neuronal cell adhesion molecule , tyrosine-protein-kinase-like 7 , and vWF ) are shown in Fig. 5A
5. Non-glycosylated proteins , such as annexin A2 and alpha-enolase , were more abundant in the flow-through
6. Glyco proteins identified in all three methods are highlighted in Fig. 5B

Output (sent_index, trigger, protein, sugar, site):

• 1. glycoprotein, , glycoprotein, -, -
• 3. glycoproteins, , glycoproteins, -, -
• 5. Non-glycosylated, , alpha-enolase, -, -
• 5. Non-glycosylated, , annexin A2, -, -
• 5. Non-glycosylated, , proteins, -, -

Output(Part-Of) (sent_index, protein, site):

*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

Protein	NCBI ID	SENTENCE INDEX