Title : Confirmation of Predicted Glycosylation Sites
Abstract :
- The hemostatic protein vWF is the main protein stored within Weibel-Palade bodies (27)
- After secretagogue stimulation, Weibel-Palade bodies undergo exocytosis, releasing vWF filaments
- vWF is one of the few known proteins containing the ABO blood group signature, which is formed by different glycans
- Although the released glycan com position of this protein has been investigated extensively (28, 29), experimental evidence for many putative glycosylation sites is still missing
- The coverage obtained for vWF in our proteomics analysis is shown in Fig. 6A
- The precursor protein consists of homologous units such as the VWF type A, C, and D domains and a C-terminal cystine know (CTCK)
- The vWF propeptide (D1-D2, AA 23–763) is separated from the remaining domains of mature vWF (AA 764–2813) via furin-mediated proteolytic cleavage
- We confirmed 6 N-glycosylation sites
- Notably, three N-glycosylation sites were located within the propeptide (AA 23–763)
- Examples of ETD spectra are shown in Fig. 6B
Output (sent_index, trigger,
protein,
sugar,
site):
- 4. glycosylation, , -, -, sites
- 8. N-glycosylation, , -, -, sites
- 9. N-glycosylation, , -, -, sites
Output(Part-Of) (sent_index,
protein,
site):
- 4. protein, position
- 7. vWF, domains
- 7. vWF, propeptide
*Output_Site_Fusion* (sent_index,
protein,
sugar,
site):