PMID: PMC3617342-1-6

 

    Legend: Gene, Sites

Title : Confirmation of Predicted Glycosylation Sites

Abstract :
  1. The hemostatic protein vWF is the main protein stored within Weibel-Palade bodies (27)
  2. After secretagogue stimulation, Weibel-Palade bodies undergo exocytosis, releasing vWF filaments
  3. vWF is one of the few known proteins containing the ABO blood group signature, which is formed by different glycans
  4. Although the released glycan com position of this protein has been investigated extensively (28, 29), experimental evidence for many putative glycosylation sites is still missing
  5. The coverage obtained for vWF in our proteomics analysis is shown in Fig. 6A
  6. The precursor protein consists of homologous units such as the VWF type A, C, and D domains and a C-terminal cystine know (CTCK)
  7. The vWF propeptide (D1-D2, AA 23–763) is separated from the remaining domains of mature vWF (AA 764–2813) via furin-mediated proteolytic cleavage
  8. We confirmed 6 N-glycosylation sites
  9. Notably, three N-glycosylation sites were located within the propeptide (AA 23–763)
  10. Examples of ETD spectra are shown in Fig. 6B
Output (sent_index, trigger, protein, sugar, site):
  • 4. glycosylation, , -, -, sites
  • 8. N-glycosylation, , -, -, sites
  • 9. N-glycosylation, , -, -, sites
Output(Part-Of) (sent_index, protein, site):
  • 4. protein, position
  • 7. vWF, domains
  • 7. vWF, propeptide
*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

 

Protein NCBI ID SENTENCE INDEX