PMID: PMC3617342-1-fig1

 

    Legend: Gene, Sites

Title : PMA treatment to stimulate EC secretion

Abstract :

1. Treatment of HUVECs with PMA, a commonly used secretagogue, resulted in a characteristic morphological change indicative of activation
2. A, immunofluorescence staining of vWF (green) and VE-cadherin (red) shows the exocytotic effect of PMA
3. B, PMA increased protein secretion in the conditioned media as confirmed via immunoblotting
4. C, relative to previous studies, more than twice as many secreted and plasma membrane proteins were identified
5. D, overlay of intracellular and secreted proteins by means of difference gel electrophoresis
6. In the left-hand panel, proteins in conditioned media of HUVECs are stained in green (+PMA) and red (−PMA), and cellular proteins are stained in blue
7. Results were reproduced with different biological replicates using reverse-labeling (right-hand panel: red, +PMA; green, −PMA)
8. The protein corresponding to von Willebrand antigen 2 is highlighted with a box
9. Common proteins in the secretome and the cellular proteome are numbered in supplemental Fig
10. S2 and listed in supplemental Table S3

Output (sent_index, trigger, protein, sugar, site):

Output(Part-Of) (sent_index, protein, site):

*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

Protein	NCBI ID	SENTENCE INDEX