Coverage is highlighted in green, and potential glycosylation sites are shown in red
A large hexagon indicates a glycosylation site with a reference in the Uniprot database
By using the HCD-ETD (H) or PNGase F (P) method, we confirmed six N-glycosylation sites on vWF
B, ETD spectra of glycopeptides identified via HCD-ETD ( N156, N211, N666, N1574 )
The following abbreviations are used: a, y, g, k = TMT modified Ala, Tyr, Gly, and Lys , respectively; c = carboxyamidomethylation of Cys ; m = oxidation of Met