PMID: PMC3617342-2

 

    Legend: Gene, Sites

Title : This study represents a significant advance over the existing proteomics literature on ECs

Abstract :
  1. Unlike other cell types, ECs do not tolerate prolonged serum starvation, and their susceptibility to cell death upon serum withdrawal poses a major challenge for proteomic workflows targeting their secretome
  2. We performed secretome analysis after 45 min of PMA stimulation combined with enrichment strategies for glycoproteins and glycopeptides
  3. Glycopeptides were analyzed via three complementary MS techniques: the detection of 18O asparagine deamidation after digestion with PNGase F in H218O, HCD-alt-ETD , and HCD-pd-ETD using an Orbitrap Elite MS
Output (sent_index, trigger, protein, sugar, site):
  • 2. glycopeptides, , -, -, glycopeptides
  • 2. glycoproteins, , glycoproteins, -, -
Output(Part-Of) (sent_index, protein, site):
*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

 

Protein NCBI ID SENTENCE INDEX