Title : This study represents a significant advance over the existing proteomics literature on ECs
Abstract :
- Unlike other cell types, ECs do not tolerate prolonged serum starvation, and their susceptibility to cell death upon serum withdrawal poses a major challenge for proteomic workflows targeting their secretome
- We performed secretome analysis after 45 min of PMA stimulation combined with enrichment strategies for glycoproteins and glycopeptides
- Glycopeptides were analyzed via three complementary MS techniques: the detection of 18O asparagine deamidation after digestion with PNGase F in H218O, HCD-alt-ETD , and HCD-pd-ETD using an Orbitrap Elite MS
Output (sent_index, trigger,
protein,
sugar,
site):
- 2. glycopeptides, , -, -, glycopeptides
- 2. glycoproteins, , glycoproteins, -, -
Output(Part-Of) (sent_index,
protein,
site):
*Output_Site_Fusion* (sent_index,
protein,
sugar,
site):