Title : The Endothelial Secretome
Abstract :
- The secretagogue PMA minimized EC death by allowing a shorter incubation period under serum-free conditions while increasing coverage in the proteomic analysis by inducing the exocytosis of intracellular storage vesicles (14) such as Weibel-Palade bodies
- These unique storage vesicles in ECs play a major role in hemostasis and cell-to-cell communication
- Using this approach, many more proteins were identified than in any previous proteomics study on ECs, including known endothelial surface markers such as endoglin ( CD105 ), integrin beta-1 ( CD29 ), tyrosine-protein kinase receptor Tie-1, and junctional adhesion molecule A ; secreted growth factors (i.e. C-type lectin domain family 11 member A ); co-receptors (i.e. neuropilin-1 ( co-receptor for VEGF-A )); proteases(i.e. furin ); and inflammatory mediators (i.e. macrophage migration inhibitory factor ), to name just a few
- Short-term PMA treatment does not release microparticles (30), as shedding events make it difficult to discern intracellular from secreted/membrane proteins
- In a direct comparison of the cellular proteome and the secretome utilizing difference gel electrophoresis, 70 out of 96 proteins analyzed were present in both samples, representing <10% of the visible protein spots in the secretome
Output (sent_index, trigger,
protein,
sugar,
site):
Output(Part-Of) (sent_index,
protein,
site):
*Output_Site_Fusion* (sent_index,
protein,
sugar,
site):