PMID: PMC3975653-1-1

 

    Legend: Gene, Sites

Title : ProteomicsAnalysis

Abstract :
  1. Tryptic peptides were digestedfrom HDL fractions isolated by ultracentrifugation
  2. After high-performanceliquid chromatography (HPLC) separation via reversed phase C18 column,the peptides were analyzed by tandem MS. Following a database search,17 proteins were identified shown in SupplementalTable S1, Supporting Information from one replicate as an example
  3. The base −10 log of the expectation that any particular proteinassignment was made at random was lower than −2
  4. ApoA-I wasobserved as the major protein
  5. In humans, about 60% of the proteincontent in HDL is represented by ApoA-I
  6. Other apoproteins were present, including Apo C-II, C-III, D, E,and M, which are typical minor apoproteins associated with HDL
  7. Onthe other hand, ApoB-100, the major constituent of VLDL and LDL, wasnot present
  8. Other proteins known to be associated with HDL were alsoobserved, including acute phase response proteins such as SAA , liversecreted proteins such as alpha-2-HS-glycoprotein ( fetuin A ), andthe key mediators of the complement system C3 inhibitor
  9. The proteins associated with HDL isolated from healthy human plasmain this study have all been suggested as HDL-associated proteins inprevious studies
  10. These results confirmed our HDL isolation approach and provideda list of proteins for site-specific glycosylation analysis
Output (sent_index, trigger, protein, sugar, site):
  • 8. alpha-2-HS-glycoprotein, , alpha-2-HS-glycoprotein, -, -
  • 8. alpha-2-HS-glycoprotein, , fetuin A, -, -
Output(Part-Of) (sent_index, protein, site):
*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

 

Protein NCBI ID SENTENCE INDEX