PMID: PMC4084840-1-4

 

    Legend: Gene, Sites

Title : N-Glycan Microheterogeneity in Serum-Derived ITIH4

Abstract :
  1. Analysis of serum-derived ITIH4 glycopeptides showedthat all canonical glycosylation sites in serum-derived ITIH4 containedcomplex sialylated N-linked glycans (Table 3)
  2. Analysis of detached, permethylated N-linked glycans from serum-derived ITIH4 was consistent with glycopeptide results (data not shown)
  3. Glycosylationsites N81 and N207 , located closest to the N-terminus of the protein,carried primarily bi- and triantennary sialylated glycans; a higherdegree of microheterogeneity was present at the N517 and N577 siteswith additional fucosylated and tetra-antennary sialylated glycansobserved in addition to the dominant bi- and triantennary sialylatedforms
  4. We detected the highest number of glycoforms, and highly branchedtetra-antennary glycans, at the N517 site
  5. A CID fragmentation spectrumof [LPTQNITFQTE + A2G2S2 + 4H]4+ (m/z 874.9) glycopeptide is shown in Figure 2A
  6. Oxonium ions, including [HexNAc + H]1+ at m/z 204, [NeuAc – H2O + H]1+ at m/z 274, [Neu5Ac + H]1+ at m/z 292, [HexNAc-Hex + H]1+ at m/z 366 and [HexNAc-Hex-Neu5Ac + H]1+ at m/z 657 in the spectrum are diagnosticof glycopeptide fragmentation and offer clues regarding glycan topology;intact peptide peaks including [LPTQNITFQTE + H]1+ at m/z 1291.65, and [LPTQNITFQTE+ HexNAc + H]1+ at m/z 1494.73 enable the assignment of peptide identity and the determinationof the N-linked glycan com position ; and peptide b and y ions (y2: m/z 249.11, 1+; b4: m/z 440.25, 1+; b5: m/z 554.29, 1+; b9: m/z 1043.54, 1+; and b10: m/z 1144.59,1+) corroborate the identity of the peptide
Output (sent_index, trigger, protein, sugar, site):
  • 1. glycopeptides, , -, -, glycopeptides
  • 1. glycosylation, , -, -, sites
  • 2. glycopeptide, , -, -, glycopeptide
  • 3. fucosylated, , -, -, N517 and N577 siteswith
  • 3. sialylated, , -, -, N517 and N577 siteswith
  • 5. glycopeptide, , -, -, glycopeptide
  • 6. glycopeptide, , -, -, glycopeptide
  • 6. identity, , -, -, position
Output(Part-Of) (sent_index, protein, site):
  • 1. ITIH4, glycopeptides
*Output_Site_Fusion* (sent_index, protein, sugar, site):
  • 3. ITIH4, -, N517 and N577 siteswith

 

 

Protein NCBI ID SENTENCE INDEX