Title : Purification of glycoprotein ITIH4 from human serum
Abstract :
(A) Purificationschema of ITIH4 from serum
(B) Western blot (anti- ITIH4 , SC-21987)of ITIH4 overexpressed and purified from HEK293 cells ( Cell ITIH4 )and from serum (serum ITIH4 )
Lane 1: Cell-derived ITIH4 ; lane 2:Cell ITIH4 treated with PNGaseF; lane 3: protein ladder with proteinmolecular weights indicated to the left of the blot; lane 4: serum ITIH4 ; lane 5: serum ITIH4 treated with PNGaseF