Title : Canonical
ITIH4 N-Glycosylation Sites areHighly Occupied
Abstract :
- We have determined occupancy of each glycosylationsite quantitatively by treatment of the N-glycopeptides by PNGaseFin the presence of H218O
- Our results show thatat least three of the four canonical N-glycosylation sites ( N207,N517 N207,N517, and N577 ) are highly occupied >90% occupancy) in serum-derived ITIH4 , and a similarly high degree of glycosylation was observed onthe recombinant ITIH4
- We also confirm that site N81 is glycosylated
- N-Glycosylation site occupancy is thought to depend on the primarysequence of the protein because the transfer of precursor glycansfrom dolichol donors to asparagine residues occurs prior to proteinfolding
- The observed similarity in occupancy betweenrecombinant and serum-derived ITIH4 is consistent with this hypothesisand suggests that cell culture might be a representative model fordetermination of site occupancy of protein glycoforms
- However, variationsin cell culture conditions, including changes in temperature, pH,and the availability of metal ions, reportedly affect N-glycosylationsite occupancy
- It is interesting tonote that the noncanonical glycosylation site at position N274 haslow occupancy in both recombinant and serum-derived ITIH4
Output (sent_index, trigger,
protein,
sugar,
site):
- 1. N-glycopeptides, , -, -, N-glycopeptides
- 1. occupancy, , -, -, glycosylationsite
- 2. N-glycosylation, , -, -, N207,N517, and N577
- 2. N-glycosylation, , -, -, N207,N517
- 2. N-glycosylation, , -, -, sites
- 2. occupied, , -, -, N207,N517, and N577
- 2. occupied, , -, -, N207,N517
- 2. occupied, , -, -, sites
- 2. sites, , -, -, N207,N517, and N577
- 2. sites, , -, -, N207,N517
- 2. sites, , -, -, sites
- 3. glycosylated, , -, -, site N81
- 5. glycoforms, , protein, -, -
- 5. occupancy, , -, -, site
- 7. glycosylation, , -, -, position N274
- 7. glycosylation, , -, -, site
Output(Part-Of) (sent_index,
protein,
site):
*Output_Site_Fusion* (sent_index,
protein,
sugar,
site):
- 2. ITIH4, -, N207,N517, and N577
- 2. ITIH4, -, N207,N517
- 3. ITIH4, -, site N81
- 7. ITIH4, -, position N274