PMID: PMC4084840-2-1

 

    Legend: Gene, Sites

Title : Canonical ITIH4 N-Glycosylation Sites areHighly Occupied

Abstract :
  1. We have determined occupancy of each glycosylationsite quantitatively by treatment of the N-glycopeptides by PNGaseFin the presence of H218O
  2. Our results show thatat least three of the four canonical N-glycosylation sites ( N207,N517 N207,N517, and N577 ) are highly occupied >90% occupancy) in serum-derived ITIH4 , and a similarly high degree of glycosylation was observed onthe recombinant ITIH4
  3. We also confirm that site N81 is glycosylated
  4. N-Glycosylation site occupancy is thought to depend on the primarysequence of the protein because the transfer of precursor glycansfrom dolichol donors to asparagine residues occurs prior to proteinfolding
  5. The observed similarity in occupancy betweenrecombinant and serum-derived ITIH4 is consistent with this hypothesisand suggests that cell culture might be a representative model fordetermination of site occupancy of protein glycoforms
  6. However, variationsin cell culture conditions, including changes in temperature, pH,and the availability of metal ions, reportedly affect N-glycosylationsite occupancy
  7. It is interesting tonote that the noncanonical glycosylation site at position N274 haslow occupancy in both recombinant and serum-derived ITIH4
Output (sent_index, trigger, protein, sugar, site):
  • 1. N-glycopeptides, , -, -, N-glycopeptides
  • 1. occupancy, , -, -, glycosylationsite
  • 2. N-glycosylation, , -, -, N207,N517, and N577
  • 2. N-glycosylation, , -, -, N207,N517
  • 2. N-glycosylation, , -, -, sites
  • 2. occupied, , -, -, N207,N517, and N577
  • 2. occupied, , -, -, N207,N517
  • 2. occupied, , -, -, sites
  • 2. sites, , -, -, N207,N517, and N577
  • 2. sites, , -, -, N207,N517
  • 2. sites, , -, -, sites
  • 3. glycosylated, , -, -, site N81
  • 5. glycoforms, , protein, -, -
  • 5. occupancy, , -, -, site
  • 7. glycosylation, , -, -, position N274
  • 7. glycosylation, , -, -, site
Output(Part-Of) (sent_index, protein, site):
*Output_Site_Fusion* (sent_index, protein, sugar, site):
  • 2. ITIH4, -, N207,N517, and N577
  • 2. ITIH4, -, N207,N517
  • 3. ITIH4, -, site N81
  • 7. ITIH4, -, position N274

 

 

Protein NCBI ID SENTENCE INDEX